HEL-311 [HEL-31]

80022 ^™

Organism: Homo sapiens, human
Clone type: Clone
Applications: Molecular biology
Product format: Freeze-dried

Documentation

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Characteristics

Comments: Restriction digests of the clone give the following sizes (kb): BamHI--3.05, 1.85, 1.25; EcoRI--2.55, 1.85 (doublet); PstI--2.9, 1.1, 0.67, 0.60, 0.32; PvuII--2.25, 1.85, 1.5, 0.8; XbaI--4.0, 1.85, 0.30.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.68; NdeI--1.10; PstI--0.47, 0.75, 1.29; PvuII--0.56; SphI--1.52; XbaI--1.65.
The insert is an alternatively spliced product containing exon 2b in the 5' untranslated region.
Contains two inserts, tandemly repeated.
Oriented with the 5' end closer to the HindIII site of the polylinker.
Mycoplasma contamination: Not detected

Vector information

Construct size (kb): 6.300000190734863
Intact vector size: 2.686
Vector name: pUC18
Type of vector: plasmid
Construction: pUC71K
Host range: Escherichia coli
Vector end: EcoRI
Vector information: Cross references: DNA Seq. Acc.: L08752
Cloning sites: HindIII; SphI; PstI; SalI; AccI; HincII; XbaI; BamHI; SmaI; KpnI; SacI; EcoRI
Insert detection: lacZ', <-
Markers: ampR
MCS: HindIII...EcoRI, ->
Polylinker sites: HindIII; SphI; PstI; SalI; AccI; HincII; XbaI; BamHI; SmaI; KpnI; SacI; EcoRI
Promoters: lac
Replicon: pMB1, <-

Insert information

Insert size (kb): 1.8
Type of DNA: cDNA
Insert source: erythroid leukemia (HEL) cell line
Insert tissue: erythroid leukemia (HEL) cell line
Insert information: DESCRIPTION OF INSERT COMPONENT:
receptor)
Cross references: DNA Seq. Acc.: L06850
Nucleotides 1-1820 of the insert correspond to
nucleotides 1-1820 of L06850.
Genome: Homo sapiens
Chromosome: 7

7 q11.2
Gene name: CD36 antigen (collagen type I receptor, thrombospondin receptor) - like 1
Gene product: CD36 antigen (collagen type I receptor, thrombospondin receptor) [CD36]
Gene symbol: CD36
Contains complete coding sequence: Yes
Insert end: EcoRI

Handling information

Host: Escherichia coli HB101 (ATCC 33694)
Medium: ATCC Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Temperature: 37°C
Handling notes: Restriction digests of the clone give the following sizes (kb): BamHI--3.05,
1.85, 1.25; EcoRI--2.55, 1.85 (doublet); PstI--2.9, 1.1, 0.67, 0.60, 0.32;
PvuII--2.25, 1.85, 1.5, 0.8; XbaI--4.0, 1.85, 0.30. Contains two inserts,
tandemLy repeated.
- ATCC staff

Oriented with the 5' end closer to the HindIII site of the polylinker.
- personal communication

The insert is an alternatively spliced product containing exon 2b in the 5'
untranslated region.
- Gene 133: 205-212, 1993

The insert contains the following restriction sites (approximate kb from the 5'
end): BamHI--0.68; NdeI--1.10; PstI--0.47, 0.75, 1.29; PvuII--0.56; SphI--1.52;
XbaI--1.65.
- Gene 133: 205-212, 1993

More information may be available from ATCC
(http://www.atcc.org or 703-365-2700).

History

Depositors: KT Taylor, RH Lipsky
Cross references: GenBank L06850

GenBank 266426

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

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Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Taylor KT, et al. Characterization of two alternatively spliced 5'-untranslated exons of the human CD36 gene in different cell types. Gene 133: 205-212, 1993. PubMed: 7693552

Kirk T Taylor, personal communication

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