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pSVLfur

79822

Organism
Homo sapiens
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Mission Collection Item
This is a Mission Collection Item

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Detailed product information

General

Specific applications
in another host, produces protein paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein) paired basic amino acid cleaving enzyme, furin, paired basic amino acid cleaving enzyme

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6, 2.4; EcoRI--4.1, 2.7, 2.2; KpnI--3.9, 3.4, 1.8; PstI--3.1, 2.5, 2.0, 1.3; SmaI--5.8, 3.4.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.48, 2.42, 3.62; KpnI--0.58, 3.97; BamHI--1.84.
Contains the complete coding sequence (nt 217-2601).
Constructed by introducing an EcoRI site in the SmaI site of the vector.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
9.0
Intact vector size
4.889
Vector name
pSVL
Type of vector
plasmid
Construction
pBR322, SV40
Host range
vertebrate cells
Vector end
EcoRI
Cloning sites
XhoI; XbaI; SmaI; XmaI; SacI; BamHI
Markers
ampR
Polylinker sites
XhoI; XbaI; SmaI; XmaI; SacI; BamHI
Promoters
SV40 late
Replicon
pMB1; SV40
Terminator
SV40 late polyadenylation

Insert information

Insert size (kb)
4.0999999999999996
Type of DNA
cDNA
Insert information
DESCRIPTION OF INSERT COMPONENT:
Genomic copy number: unique
associated receptor protein)
Cross references: DNA Seq. Acc.: X17094
Genome
Homo sapiens
Chromosome
15
15 q26.1
Target gene
paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein)
Gene name
paired basic amino acid cleaving enzyme (furin, membrane
Gene product
paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein)( paired basic amino acid cleaving enzyme, furin, paired basic amino acid cleaving enzyme) [FUR]
Gene symbol
PACE; FUR
Contains complete coding sequence
Yes
Insert end
Modification: EcoRI linkers

Handling information

Host
Escherichia coli HB101 (ATCC 33694)
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): BamHI--6.6, 2.4;
EcoRI--4.1, 2.7, 2.2; KpnI--3.9, 3.4, 1.8; PstI--3.1, 2.5, 2.0, 1.3; SmaI--5.8,
3.4.
- ATCC staff

The insert contains the following restriction sites (approximate kb from the 5'
end): PstI--0.48, 2.42, 3.62; KpnI--0.58, 3.97; BamHI--1.84.
- GenBank/EMBL/DDBJ

Corresponds to nt 101-4180 of the sequence record. Constructed by introducing an
EcoRI site in the SmaI site of the vector.
- personal communication

Contains the complete coding sequence (nt 217-2601).
- Mol. Biol. Rep. 14: 265-275, 1990


More information may be available from ATCC
(http://www.atcc.org or 703-365-2700).

History

Depositors
WJ Van De Ven
Cross references
GenBank X17094

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

van den Ouweland AM, et al. Structural homology between the human fur gene product and the subtilisin-like protease encoded by yeast KEX2. Nucleic Acids Res. 18: 664, 1990. PubMed: 2408021

Van de Ven WJ, et al. Furin: the prototype mammalian subtilisin-like proprotein-processing enzyme. Endoproteolytic cleavage at paired basic residues of proproteins of the eukaryotic secretory pathway. Enzyme 45: 257-270, 1991. PubMed: 1843280

van de Ven WJ, et al. Furin is a subtilisin-like proprotein processing enzyme in higher eukaryotes. Mol. Biol. Rep. 14: 265-275, 1990. PubMed: 2094803

Wim J Van De Ven, personal communication

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