p67-10

79080 ^™

Detects:
Genome: Homo sapiens
Gene symbol: NCF2
Type of nucleic acid: genomic
Restriction enzyme: HindIII
Invariant bands (kb): 13, 12
Number of alleles: 2
Type of polymorphism: site
Alleles:
Allele Freq. Size (kb) Strains
A1 0.80 3.8
A2 0.20 2.1

Organism: Homo sapiens, human
Clone type: Clone
Applications: Molecular biology
Product format: Freeze-dried

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Documentation

Product sheet

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

General

Specific applications: produces protein neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) neutrophil cytosolic factor 2

Characteristics

Comments: Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.1; SstI--3.7, 0.9, 0.41; BamHI--4.0, 1.0; BglII--5.1; PstI--5.1.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--0.84, 1.22; BamHI--0.97; BglII--0.78; SmaI--1.17.
Contains the complete coding sequence (nt 68-1648).
Detects a transcript of 2.4 kb.
The polymorphism was detected with a 0.84 kb EcoRI/SacI fragment.
Enzymes(s) not detecting polymorphism: AvaI, BamHI, EcoRI, KpnI, PstI, SacI, XhoI.
Mycoplasma contamination: Not detected

Vector information

Construct size (kb): 5.199999809265137
Intact vector size: 2.960
Vector name: pBluescript SK-
Type of vector: phagemid
Construction: pUC19
Host range: Escherichia coli
Vector end: EcoRI
Cloning sites: KpnI; ApaI; DraII; XhoI; SalI; HincII; AccI; ClaI; HindIII; EcoRV; EcoRI; PstI; SmaI; BamHI; SpeI; XbaI; EagI; NotI; SacII; BstXI; SacI
Insert detection: lacZ', <-
Markers: ampR
MCS: KpnI...SacI, ->
Polylinker sites: SacI; BstXI; SacII; NotI; EagI; XbaI; SpeI; BamHI; SmaI; PstI; EcoRI; EcoRV; HindIII; (ClaI Bsp106I); (HincII AccI SalI); XhoI; (Eco0109I DraII ApaI); KpnI
Primer site: M13-20, ->; M13 reverse, <-
Promoters: T3; T7
Replicon: f1, →; pMB1

Insert information

Insert size (kb): 2.1600000000000001
Type of DNA: cDNA
Insert source: HL-60 cell line (promyelocytic), differentiated
Insert tissue: HL-60 cell line (promyelocytic), differentiated
Insert information: DESCRIPTION OF INSERT COMPONENT:
Genomic copy number: unique
disease, autosomal 2)
Cross references: DNA Seq. Acc.: M32011
Genome: Homo sapiens
Chromosome: 1

1 cen-q32
Target gene: neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)
Gene name: neutrophil cytosolic factor 2 (65kD, chronic granulomatous
Gene product: neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)(neutrophil cytosolic factor 2) [NCF2]
Gene symbol: NCF2
Alleles: A1, A2
Contains complete coding sequence: Yes
Insert end: Modification: EcoRI linkers

Handling information

Host: Escherichia coli JM109 (ATCC 53323)
Medium: ATCC Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Temperature: 37°C
Handling notes: Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.1;
SstI--3.7, 0.9, 0.41; BamHI--4.0, 1.0; BglII--5.1; PstI--5.1.
- ATCC staff

Contains the complete coding sequence (nt 68-1648).
- Science 248: 727-730, 1990

Detects a transcript of 2.4 kb.
- Science 248: 727-730, 1990

The insert contains the following restriction sites (approximate kb from the 5'
end): SacI--0.84, 1.22; BamHI--0.97; BglII--0.78; SmaI--1.17.
- Science 248: 727-730, 1990

Enzymes(s) not detecting polymorphism: AvaI, BamHI, EcoRI, KpnI, PstI, SacI,
XhoI.
- Nucleic Acids Res. 18: 7193, 1990

The polymorphism was detected with a 0.84 kb EcoRI/SacI fragment.
- Nucleic Acids Res. 18: 7193, 1990

More information may be available from ATCC (http://www.atcc.org or 703-365-2620).

History

Depositors: TL Leto
Cross references: GenBank M32011

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Francke U, et al. Genes for two autosomal recessive forms of chronic granulomatous disease assigned to 1q25 (NCF2) and 7q11.23 (NCF1). Am. J. Hum. Genet. 47: 483-492, 1990. PubMed: 2393022

Leto TL, et al. Cloning of a 67-kD neutrophil oxidase factor with similarity to a noncatalytic region of p60(c-src). Science 248: 727-730, 1990. PubMed: 1692159

Kenney RT, Leto TL. A HindIII polymorphism in the human NCF2 gene. Nucleic Acids Res. 18: 7193, 1990. PubMed: 1979859

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