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lambdahSSTR2-11

79046

Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
bacteria-free lysate
Mission Collection Item
This is a Mission Collection Item.

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--26.5, 23.0, 2.0; XhoI-->23.0, 14.0; SacI--25.0, 16.5, 3.3, 2.9, 1.4, 1.2, 0.8; SalI--25.0, 15.5, 6.3, 4.4; XbaI--20.0, 9.2, 7.0, 3.9, 3.7, 2.2, 2.0, 1.8, 1.6.
Detects a murine genomic EcoRI fragment of 8.2 kb.
Additional GenBank/EMBL accession number: L05521.
Detects human transcripts of 8.5 and 2.5 kb.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
44.0
Intact vector size
41.900
Vector name
lambdaFIX II
Type of vector
phage
Host range
Escherichia coli
Vector end
XhoI
Cloning sites
NotI; SalI; XhoI; EcoRI
Polylinker sites
XbaI; SacI; NotI; SacI; SalI; XhoI; EcoRI; XbaI
Promoters
T3; T7
Replicon
lambda

Insert information

Insert size (kb)
15
Type of DNA
genomic
Insert source
placenta, male Caucasian
Insert tissue
placenta, male Caucasian
Insert information
DESCRIPTION OF INSERT COMPONENT:
Cross references: DNA Seq. Acc.: M81830
Genome
Homo sapiens
Chromosome
17
17 q24
Gene name
somatostatin receptor 2
Gene product
somatostatin receptor 2 [SSTR2]
Gene symbol
SSTR2
Contains complete coding sequence
Yes

Handling information

Handling notes
Restriction digests of the clone give the following sizes (kb): EcoRI--26.5,
23.0, 2.0; XhoI-->23.0, 14.0; SacI--25.0, 16.5, 3.3, 2.9, 1.4, 1.2, 0.8;
SalI--25.0, 15.5, 6.3, 4.4; XbaI--20.0, 9.2, 7.0, 3.9, 3.7, 2.2, 2.0, 1.8, 1.6.
- ATCC staff

A BamHI/XbaI fragment corresponds to nt 1-1293 of the GenBank record and
contains the complete coding sequence (nt 15-1124).
- Proc. Natl. Acad. Sci. USA 89: 251-255, 1992

Detects a murine genomic EcoRI fragment of 8.2 kb.
- Proc. Natl. Acad. Sci. USA 89: 251-255, 1992

Additional GenBank/EMBL accession number: L05521.
- Genomics 15: 449-452, 1993

Detects human transcripts of 8.5 and 2.5 kb.
- Proc. Natl. Acad. Sci. USA 89: 251-255, 1992


More information may be available from ATCC (http://www.atcc.org or 703-365-2620).

History

Depositors
GI Bell
Cross references
GenBank M81830

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

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Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Yamada Y, et al. Cloning and functional characterization of a family of human and mouse somatostatin receptors expressed in brain, gastrointestinal tract, and kidney. Proc. Natl. Acad. Sci. USA 89: 251-255, 1992. PubMed: 1346068

Yamada Y, et al. Human somatostatin receptor genes: localization to human chromosomes 14, 17, and 22 and identification of simple tandem repeat polymorphisms. Genomics 15: 449-452, 1993. PubMed: 8449518

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