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pUK4134

77480

Organism
Escherichia coli (Migula) Castellani and Chalmers
Classification
Enterobacteriaceae, Escherichia
Clone type
Vector
Applications
Molecular biology
Product format
Freeze-dried
Mission Collection Item
This is a Mission Collection Item.

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
contains sequence origin of transfer replication
contains sequence ribosomal protein S12
integrating vector
shuttle vector
vector permitting positive selection for integration

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.7, 0.5; EcoRI--6.4, 0.8; EcoRV--7.2; SalI--7.2.
RP6 is a suicide replicon in hosts not producing the pir protein.
RpsL (streptomycin sensitive) is dominant to streptomycin resistance, so loss of the plasmid can identified by streptomycin resistance and ampicillin sensitivity.
Suicide, gene replacement vector for Yersiniae and other gram-negative hosts that permits positive selection for loss of the plasmid after allelic exchange.
The vector also contains cos sites and oriT, and can be used for cosmid cloning or conjugative transfer.
The order of the major features in the plasmid is: EcoRV - R6K ori - oriT - SalI - cos - rpsL - ampR.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
6.699999809265137
Vector name
pUK4134 (plasmid)
Type of vector
plasmid
Construction
pRTP1, pJM703.1
Vector information
other: lambda cos site
Markers
strS; ampR
Replicon
R6K; oriT

Insert information

Type of DNA
cDNA
Target gene
ribosomal protein S12, origin of transfer replication
Gene product
origin of transfer replication [oriT]

Handling information

History

Depositors
SC Straley

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Skrzypek E, et al. New suicide vector for gene replacement in Yersiniae and other gram-negative bacteria. Plasmid 29: 160-163, 1993. PubMed: 8469722

Stibitz S, et al. The construction of a cloning vector designed for gene replacement in Bordetella pertussis. Gene 50: 133-140, 1986. PubMed: 2884169

Miller VL, Mekalanos JJ. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J. Bacteriol. 170: 2575-2583, 1988. PubMed: 2836362

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