ATCC 100 Years Logo Anniversary ATCC 100 Years Logo Anniversary Cart 0
  • Quick Order
  • Careers
  • Support

pMOB3

77282

Organism
Bacillus subtilis subsp. subtilis (Ehrenberg) Cohn
Clone type
Clone
Applications
Molecular biology
Product format
Frozen
Buy Now
Price: $639.00 EA
Discounts may be available for our fellow nonprofit organizations. Login to see your price.

Generally ships within 1-3 business days

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): NotI--5.6, 2.4; EcoRI/HindIII--2.8, 1.5, 1.15, 0.9, 0.7, 0.55, 0.2; HindIII--4.2, 1.8, 0.9, 0.8.
Available for research purposes only.
Expression of sacB confers sensitivity to sucrose (no growth on LB medium containing 5% sucrose).
Plasmid containing an oriT/cmlR/sacBR mobilization cassette that can be inserted into a general purpose vector (ATCC 77278, 77279) for conjugal transfer of cloned inserts into the chromosome of P. aeruginosa.
Constructed by inserting a 0.9 kb BamHI fragment containing cmlR into the BamHI site of pMOB2 (ATCC 77281). The NotI digest excises a fragment of approximately 5.4 kb that includes short segments of the pHSS21 sequence.
The NotI cassette includes these features: pHSS21 sequence - EcoRI - oriT - PstI - BamHI - HindIII - cmlR - HindIII - BamHI - XbaI - SalI - PstI - sacBR/HindIII/EcoRI/XbaI/SalI - PstI - SphI - HindIII - pHSS21 sequence.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
8.100000381469727
Vector name
pHSS21
Type of vector
plasmid
Construction
pUC19
Host range
Escherichia coli
Vector end
EcoRI; XhoI
Cloning sites
HindIII; SphI; SalI; XbaI; BamHI; XhoI; KpnI; SacI; EcoRI
Markers
cmlR; sacB; kanR
Polylinker sites
HindIII; SphI; PstI; SalI; XbaI; BamHI; XhoI; KpnI; SacI; EcoRI
Promoters
lac
Replicon
pMB1

Insert information

Insert size (kb)
5
Type of DNA
genomic
Genome
Bacillus subtilis; Bacillus subtilis; pCU1
Gene name
levansucrase; sucrase, levansucrase regulator; (IncN) origin of transfer replication
Gene product
(IncN) origin of transfer replication [oriT]
Gene symbol
sacB; sacR; oriT
Contains complete coding sequence
Unknown
Insert end
EcoRI; SalI

Handling information

Host
Escherichia coli DH5alphaF'
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): NotI--5.6, 2.4;
EcoRI/HindIII--2.8, 1.5, 1.15, 0.9, 0.7, 0.55, 0.2; HindIII--4.2, 1.8, 0.9, 0.8.
- ATCC staff

Available for research purposes only.
- personal communication

Plasmid containing an oriT/cmLR/sacBR mobilization cassette that can be inserted
into a general purpose vector (ATCC 77278, 77279) for conjugal transfer of
cloned inserts into the chromosome of P. aeruginosa.
- Mol. Microbiol. 6: 1195-1204, 1992

Expression of sacB confers sensitivity to sucrose (no growth on LB medium
containing 5% sucrose).
- Mol. Microbiol. 6: 1195-1204, 1992

Constructed by inserting a 0.9 kb BamHI fragment containing cmLR into the BamHI
site of pMOB2 (ATCC 77281). The NotI digest excises a fragment of approximately
5.4 kb that includes short segments of the pHSS21 sequence.
- Mol. Microbiol. 6: 1195-1204, 1992

The NotI cassette includes these features: pHSS21 sequence - EcoRI - oriT - PstI
- BamHI - HindIII - cmLR - HindIII - BamHI - XbaI - SalI - PstI -
sacBR/HindIII/EcoRI/XbaI/SalI - PstI - SphI - HindIII - pHSS21 sequence.
- Mol. Microbiol. 6: 1195-1204, 1992

History

Depositors
HP Schweizer

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Schweizer HP. Allelic exchange in Pseudomonas aeruginosa using novel ColE1-type vectors and a family of cassettes containing a portable oriT and the counter-selectable Bacillus subtilis sacB marker. Mol. Microbiol. 6: 1195-1204, 1992. PubMed: 1588818

For product-related inquiries and issues, contact Product Experience:

Message Us

Hours of Operation

Monday - Friday
9:00am - 5:00pm
US Eastern Time