75170 ™
This is a clone of the Haemophilus influenza biogroup aegyptius modification methylase and restriction endonuclease HinPI genes in the pBR322 vector.The insert contains the following restriction sites (approximate kb from the 5’ end): BglII – 1.0; ClaI – 0.9; HindIII – 5.4; PstI – 1.5; PvuII – 0.5, 5.5.
Total size of construct (kb) : 10.5
Marker : tetR
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Rehydrate plasmid with water or TE buffer. Plasmid can be transformed into a suitable Escherichia coli host using standard protocols and then grown on LB + 15 µg/mL of tetracycline agar. Incubate cultures at 37°C. Isolate DNA using standard plasmid preparation procedures.
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Barsomian JM, Wilson GG. Method for producing the HinPI restriction endonuclease and methylase. US Patent 4,983,522 dated Jan 8 1991
Barsomian JM, et al. Cloning of the HhaI and HinPI restriction-modification systems. Gene 74: 5-7, 1988. PubMed: 3074018
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