Legionella drozanskii Adeleke et al.
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2. Using a single tube of #1099 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an agar slant and/or plate.
5. Incubate at 30oC in an atmosphere of 5% CO2 for 48-72 hours.
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Adeleke A, et al. Legionella-like amebal pathogens--phylogenetic status and possible role in respiratory disease. Emerg. Infect. Dis. 2: 225-230, 1996. PubMed: 8903235
Adeleke AA, et al. Legionella drozanskii sp. nov., Legionella rowbothamii sp. nov. and Legionella fallonii sp. nov.: three unusual new Legionella species. Int. J. Syst. Evol. Microbiol. 51: 1151-1160, 2001. PubMed: 11411684