Comamonas denitrificans Gumaelius et al.
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2. From a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.6 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Use 0.5ml of this suspension to inoculate a #3 slant and 0.1ml to inoculate #3 plates.
4. Incubate tubes and plates at 30oC, under aerobic conditions, for 48 hours.
5. After 48 hours of incubation, wash cells from the slant and transfer this broth to a new slant and plate. Incubate another 48 hours under aerobic conditions. This second transfer and incubation is necessary for complete removal of the cryoprotectant, which can inhibit growth.
1 mm diameter with irregular margins and a translucent intrastructure. Cells are motile, curved bacilli.
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Gumaelius L, et al. Comamonas denitrificans sp. nov., an efficient denitrifying bacterium isolated from activated sludge. Int. J. Syst. Evol. Microbiol. 51: 999-1006, 2001. PubMed: 11411726
activated sludge in a municipal wastewater treatment plant, Gustavsberg