Erwinia toletana Rojas et al. 2004
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
5. Incubate the tubes and plate at 26-28°C for 24-48 hours.
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Rojas AM, et al. Erwinia toletana sp. nov., associated with Pseudomonas savastanoi-induced tree knots. Int. J. Syst. Evol. Microbiol. 54(Pt 6): 2217-2222. PubMed: 15545461