Pseudomonas mandelii Verhille et al.
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2. From a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate a second tube of broth, a slant, and/or a plate.
5. Incubate tubes and plate at 30oC for 24 hours.
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Verhille S, et al. Taxonomic study of bacteria isolated from natural mineral waters: proposal of Pseudomonas jessenii sp. nov. and Pseudomonas mandelii sp. nov.. Syst. Appl. Microbiol. 22: 45-58, 1999. PubMed: 10188278