Bacillus horti Yumoto et al.
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2. From a tube of #2162 broth (5 to 6 ml), withdraw approximately 0.5 with a sterile pipette; add this medium to the vial to rehydrate the pellet.
3. Aseptically transfer the contents of the vial into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #2162 agar slant and/or plate.
5. Incubate all tubes and plate at 30°C for 24-48 hours.
Growth in #2162 broth produces an abundant, flocculent sediment with transient turbidity.
Cultivability is effluent at 24-48 h.
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