Lactobacillus nagelii Edwards et al.
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2. Using a single tube of #416 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate all tubes and plate at 30°C for 24 to 48 hours.
Incubation in an atmosphere of 5% CO2 is not required but will enhance growth.
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Edwards CG, et al. Lactobacillus nagelii sp. nov., an organism isolated from a partially fermented wine. Int. J. Syst. Evol. Microbiol. 50: 699-702, 2000. PubMed: 10758877