Pseudomonas multiresinivorans Mohn et al.
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2. Using a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #18 agar slant and/or plate.
5. Incubate all tubes and plate at 30°C for 24 hours.
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Validation list no. 70. Int. J. Syst. Bacteriol. 49: 935-936, 1999.
Wilson AE, et al. Isolation and characterization of isopimaric acid-degrading bacteria from a sequencing batch reactor. Appl. Environ. Microbiol. 62: 3146-3151, 1996. PubMed: 8795202
Mohn WW, et al. Physiological and phylogenetic diversity of bacteria growing on resin acids. Syst. Appl. Microbiol. 22: 68-78, 1999. PubMed: 10188280