Pseudomonas abietaniphila Mohn et al.
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2. Using a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #18 agar slant and/or plate.
5. Incubate all tubes and plate at 30°C for 24 hours.
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Validation list no. 70. Int. J. Syst. Bacteriol. 49: 935-936, 1999.
Bicho PA, et al. Growth, induction, and substrate specificity of dehydroabietic acid-degrading bacteria isolated from a kraft mill effluent enrichment. Appl. Environ. Microbiol. 61: 3245-3250, 1995. PubMed: 7574634
Mohn WW, et al. Physiological and phylogenetic diversity of bacteria growing on resin acids. Syst. Appl. Microbiol. 22: 68-78, 1999. PubMed: 10188280
aerated lagoon of bleached kraft pulp mill effluent, Kamloops