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Helicobacter pylori (Marshall et al.) Goodwin et al.

700392

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Product category
Bacteria
Strain designation
26695 [KE26695]
Type strain
No
Genome sequenced strain
Yes
Isolation source
Stomach of patient with gastritis
Geographical isolation
United Kingdom
Applications
Bioinformatics
Enteric disease research
Infectious disease research
Product format
Frozen
Storage conditions
-80°C or colder
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Enteric Research
Genome sequencing strain
Preceptrol
No

Characteristics

Comments

Component of ATCC MSA-1002, ATCC MSA-1003, ATCC MSA-1006, ATCC MSA-2002, and ATCC MSA-2006.

Handling information

Medium
Temperature
37°C
Atmosphere
Microaerophilic: 3-5% O2, 10% CO2
Handling procedure
  1. This organism is shipped frozen in dry ice.  Just prior to use, thaw vial in water at approximately 37°C.  When thawed, a drop of the suspension may be used to do an immediate wet mount to observe the unique morphology of this organism and verify its viability by checking for motility.
  2. Aseptically transfer the thawed suspension into a fresh #18 broth (3-5 mL).  Mix well.  This suspension can now be used to inoculate agar slant(s), plate(s), or the preferred biphasic culture.  Two #260 plates should be inoculated, one for microaerophilic growth and the second for aerobic growth.  No growth should occur on the plate incubated aerobically.
  3. To obtain a biphasic culture, add 0.6 mL of the suspension to a #260 slant.  The resulting pool at the bottom of the slant is where the best, most rapid growth will occur.
  4. Incubate at 37°C under microaerophilic conditions using an anaerobe jar with an active catalyst and a microaerophilic gas generator pack, or other acceptable method, to obtain microaerophilic conditions.  Incubate tubes with cap loose.
  5. Within 2-3 days, good growth should be obtained in the broth pool at the bottom of the slant and on the microaerophilic plate.  Further subcultures can be made using the broth pool as the inoculum source.  Subcultures to biphasic cultures will require only 24 to 48 hours of incubation for good growth.

Handling notes
Growth at the broth/agar interface of the biphasic slant should occur within 3 days, but only light turbidity will be seen. To observe growth, examine a wet mount of the broth under phase microscopy. 

 

Growth on agar takes longer than the biphasic culture. The cells do not Gram stain well using traditional procedures. For best results, use a basic fuchsin counterstain in place of the safranin.

 

Once good growth in obtained, transfer or freeze the culture. Adding an equal amount of 20% sterile glycerol to pooled broth from several biphasic slants, followed by freezing in liquid nitrogen or “ultra-low temperature” freezer is recommended. 

 

Purified genomic DNA of this strain is available as ATCC® 700392D-5™.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Helicobacter pylori (Marshall et al.) Goodwin et al.
Depositors
KA Eaton
Chain of custody
ATCC <-- KA Eaton <-- AD Pearson
Type of isolate
Human
Special collection
NCRR Contract
Cross references
GenBank AE000511 Helicobacter pylori 26695, complete genome.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Tomb JF, et al. The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 388: 539-547, 1997. PubMed: 9252185

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