Lactobacillus kunkeei Edwards et al.
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2. Using a single tube of #2067 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #2067 agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 24-48 hours.
Colonies on #2067 agar are pinpoint, circular, entire, and smooth. Very poor growth resulted on MRS Agar, sheep blood agar, or Trypticase Soy Agar.
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Edwards CG, et al. Lactobacillus kunkeei sp. nov.: a spoilage organism associated with grape juice fermentations. J. Appl. Microbiol. 84: 698-702, 1998. PubMed: 9674120
Huang YC, et al. Relationship between sluggish fermentations and the antagonism of yeast by lactic acid bacteria. Am. J. Enol. Vitic. 47: 1-10, 1996.
Validation list no. 67. Int. J. Syst. Bacteriol. 48: 1083-1084, 1998.