Virgibacillus salexigens (Garabito et al.) Heyrman et al.
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2. Using a single tube of #1689 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #1689 agar slant and/or plate.
5. Incubate the tubes and plate at 37°C for 24 hours.
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Garabito MJ, et al. Bacillus salexigens sp. nov., a new moderately halophilic Bacillus species. Int. J. Syst. Bacteriol. 47: 735-741, 1997. PubMed: 9226905
Waino M, et al. Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosaurito Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. . Int. J. Syst. Bacteriol. 49: 821-831, 1999. PubMed: 10319508