Streptococcus difficilis Eldar et al.
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2. Using a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #44 agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 48 hours.
Colonies on #44 agar are entire, glistening and smooth.
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. Collection of bacterial strains. 6th ed.Paris: Institut Pasteur; 1994.