Bacillus gibsonii Nielsen et al.
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2. Using a single tube of #688 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #688 slant, and/or plate
5. Incubate all tubes and plates at 30oC for 24 to 48 hours.
6. After growth has been achieved, transfers may be made to secondary tubes of broth, slants, and /or plates.
On #688 plates colonies are round, entire, opaque, glistening, raised, smooth, and yellow.
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Validation list no. 55. Int. J. Syst. Bacteriol. 45: 879-880, 1995.
Nielsen P, et al. Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141: 1745-1761, 1995.