Bacillus agaradhaerens Nielsen et al.
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2. From a tube of #2030 broth (5 to 6 ml), withdraw approximately 0.5 with a sterile pipette; add this medium to the vial to rehydrate the entire pellet.
3. Aseptically transfer the contents of the vial into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #2030 agar slant and/or plate.
5. Incubate all tubes and plate at 30°C for 24 to 48 hours.
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Validation list no. 55. Int. J. Syst. Bacteriol. 45: 879-880, 1995.
Nielsen P, et al. Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141: 1745-1761, 1995.