Bacillus carboniphilus Fujita et al.
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2. From a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, an agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 24 to 48 hours.
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Fujita T, et al. Description of Bacillus carboniphilus sp. nov.. Int. J. Syst. Bacteriol. 46: 116-118, 1996.