Mycobacterium conspicuum Springer et al.
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2. From a single tube of the recommended broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Heavily inoculate a slant of #90 and/or a plate of #173 agar.
5. After growth has been achieved, additional transfers may be made.
Colonies on #173 plates are small, clear, entire, circular, convex, and smooth.
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Validation list no. 56. Int. J. Syst. Bacteriol. 46: 362-363, 1996.
Springer B, et al. Mycobacterium conspicuum sp. nov., a new species isolated from patients with disseminated infections. J. Clin. Microbiol. 33: 2805-2811, 1995. PubMed: 8576323