Mycobacterium triplex Floyd et al.
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2. Using a single tube of #1395 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate #90 or #173 agar slant and/or plate.
5. Incubate the tubes and plate at 37°C for 4 to 6 weeks.
Colonies on #173 agar are entire, glistening, circular, smooth, convex, and size-variable.
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