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pGK51 [pGSK51]


Escherichia coli (Migula) Castellani and Chalmers
Enterobacteriaceae, Escherichia
Clone type
Molecular biology
Product format
Currently Unavailable
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information


Specific applications
produces protein heat-labile enterotoxin
produces protein heat-stable enterotoxin


Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.686, 0.911; EcoRI/HindIII/SstI--2.686, 0.505, 0.406.
Sonicated extracts of E. coli HB101 transformed with this construct express both ST and LTB immunoreactivity.
The 0.406 kb EcoRI/SstI fragment encodes the heat stable enterotoxin, and the 0.505 kb SstI/HindIII fragment encodes the heat labile enterotoxin.
The latter fragment, derived from EWD299 (ATCC 37218), includes the coding region for the B subunit and 3' transcription and translation stop signals, but lacks the ribosome binding site and sequences encoding the signal peptide.
The former fragment is at the 5' end and includes the complete coding sequence and 5' regulatory regions (transcription initiation and ribosome binding site) but not the 3' transcription and translation stop signals.
Mycoplasma contamination
Not detected
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Vector information

Construct size (kb)

Insert information

Insert size (kb)
Type of DNA
Target gene
heat-labile enterotoxin; heat-stable enterotoxin
Gene product
heat-stable enterotoxin [lt]

Handling information


University of Texas Health Science Center, YM Kupersztoch, University of Texas Health Science Center
Patent depository
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC.  As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Guzman-Verduzco LM, Kupersztoch YM. Fusion of Escherichia coli heat-stable enterotoxin and heat-labile enterotoxin B subunit. J. Bacteriol. 169: 5201-5208, 1987. PubMed: 3312169

Guzman-Verduzco LM, Kupersztoch YM. Rectification of two Escherichia coli heat-stable enterotoxin allele sequences and lack of biological effect of changing the carboxy-terminal tyrosine to histidine. Infect. Immun. 57: 645-648, 1989. PubMed: 2643580

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