pJP1 plasmid in Bacillus sphaericus

1. Open vial according to instructions.
2. Asceptically add 0.3 to 0.4 mL of liquid medium to the freeze-dried pellet and mix well.  Transfer 100 µL to a test tube containing 5 mLVY Medium (*see below) + erythromycin (5 µg/mL). A loopful of culture can also be streaked on an agar plate of the same. Incubate cultures at 30°C.
3. Isolate DNA using standard plasmid preparation procedures for Bacillus sp.

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