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pJP1 plasmid in Bacillus sphaericus

67080

This clone contains the complete coding sequence (nt 245-1414 of Genbank M15686) of the gene lysostaphin from Staphylococcus simulans. It was constructed by excising the insert of pRG5 (ATCC 67076) with an EcoRI/HindIII digest, and blunt-ending the vector and insert with the Klenow fragment of DNA polymerase before ligation.
– U.S. Patent 4,931,390 dated June 5, 1990.
Organism
Staphylococcus simulans Kloos and Schleifer
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
B. sphaericus containing the plasmid
Storage conditions
2°C to 8°C
Mission Collection Item
This is a Mission Collection Item.

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
Produces protein lysostaphin

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--8.0; PstI--uncut; SphI--8.0.
The insert contains the following restriction sites (approximate kb from the 5' end): PvuII--0.43, 0.46, 0.50, 0.58, 0.62; SphI--1.23.
Constructed by excising the insert of pRG5 (ATCC 67076) with an EcoRI/HindIII digest, and blunt-ending the vector and insert with the Klenow fragment of DNA polymerase before ligation.
Contains the complete coding sequence (nt 245-1414 of the sequence record).
Mycoplasma contamination
Not detected
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Vector information

Construct size (kb)
8.0
Intact vector size
4.6
Vector name
pBC16
Type of vector
plasmid
Vector end
EcoRI
Markers
eryR

Insert information

Insert size (kb)
1.5
Insert information
Gene: lysostaphin
Source: Staphylococcus simulans, NRRL B-2628
Insert ends: (5’) EcoRI ; (3 ‘) HindIII
Genbank accession: M15686
Insert end
3' HindIII; 5' EcoR

Handling information

Host
Bacillus sphaericus
Medium
Handling procedure
  1. Open vial according to instructions.
  2. Asceptically add 0.3 to 0.4 mL of liquid medium to the freeze-dried pellet and mix well.  Transfer 100 µL to a test tube containing 5 mLVY Medium (*see below) + erythromycin (5 µg/mL). A loopful of culture can also be streaked on an agar plate of the same. Incubate cultures at 30°C.
  3. Isolate DNA using standard plasmid preparation procedures for Bacillus sp.
Handling notes
Restriction digests of the clone gave the following sizes (in kb): EcoRI/HindIII – 8.0 ; PstI – uncut ; Sph – 8.9.
–ATCC Staff

History

Depositors
Public Health Research Institution, PA Recsei, Public Health Research Institution
Patent depository
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC.  As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Patent number
4,931,390
Cross references
GenBank M15686

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Disclosures
This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Recsei PA. Expression of the cloned lysostaphin gene. US Patent 4,931,390 dated Jun 5 1990

Recsei PA, et al. Cloning, sequence, and expression of the lysostaphin gene from Staphylococcus simulans. Proc. Natl. Acad. Sci. USA 84: 1127-1131, 1987. PubMed: 3547405

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