Xanthophyllomyces dendrorhous Golubev
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2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25º C) undisturbed for at least 2 hours; overnight rehydration is recommended.
5. Mix the suspension well. Use several drops to inoculate appropriate solid or liquid media (see atcc.org for a recommended medium and temperature).
6. Incubate cultures at appropriate temperature. Remaining suspension may be stored in refrigerator for possible reuse, although no survival time can be specified.
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An GH, et al. Isolation of Phaffia rhodozyma mutants with increased astaxanthin content. Appl. Environ. Microbiol. 55: 116-124, 1989.
Schreiber D, et al. Processes for in vivo production of astaxanthin and Phaffia rhodozyma yeast of enhanced astaxanthin content. US Patent 5,182,208 dated Jan 26 1993
Johnson EA, et al. Processes for in vivo production of astaxanthin and Phaffia rhodozyma yeast of enhanced astaxanthin content. US Patent 5,356,809 dated Oct 18 1994