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Quantitative Genomic DNA from Staphylococcus aureus subsp. aureus strain FDA 209

6538DQ

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ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
Product category
Bacteria
Product type
Nucleic acid
Molecular standard
Derived from
Staphylococcus aureus subsp. aureus FDA 209 (ATCC 6538)
Organism
Staphylococcus aureus subsp. aureus Rosenbach
Type strain
No
Applications
Assay development
Bioinformatics
Drug development
Enteric disease research
Food testing
Infectious disease research
Media testing
Next-generation sequencing
Quality control
Water testing
Specification range
≥1 x 105 copies/μL
Volume
100 μL
Product format
Frozen
Storage conditions
-20°C or colder

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications

ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.

Characteristics

Comments
Genomic DNA isolated from a preparation of Staphylococcus aureus subsp. aureus strain FDA 209 (ATCC® 6538™). The source organism is also available through the ATCC catalog.

This strain is recommended by ATCC for use in the tests described in military specification MIL G-13734B where only the taxon is specified.

Handling information

Handling procedure
  1. Thaw the vial at room temperature and immediately place on ice. Avoid exposing the DNA to repeated freeze-thaw cycles as it may result in degradation.
  2. Gently mix the sample to ensure an even distribution of material.
  3. Briefly centrifuge the tube before opening to ensure all liquid is at the bottom.
Handling notes
Aliquoting is highly recommended to avoid multiple freeze-thaws.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Depositors
ATCC
Cross references
GenBank DQ212950 Staphylococcus aureus strain ATCC 6538 16S ribosomal RNA gene, partial sequence.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Frequently Asked Questions

References

Curated Citations

Connolly P, et al. The use of impedance for preservative efficacy testing of pharmaceuticals and cosmetic products. J. Appl. Bacteriol. 76: 68-74, 1994. PubMed: 8144407

ASTM International StandardTest Method for Efficacy of Sanitizers Recommended for Inanimate Non-Food Contact Surfaces. West Conshohocken, PA

ASTM International Standard Test Method for Preservatives in Water-Containing Cosmetics. West Conshohocken, PA

AOAC International Germicidal and detergent sanitizing action of disinfectants. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 960.09.

AOAC International Bacteriostatic activity of laundry additive disinfectants. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 972.04.

View All Curated Citations for this Product

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