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Genomic DNA from Staphylococcus aureus subsp. aureus strain FDA 209

6538D-5

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Genomic DNA isolated from Staphylococcus aureus subsp. aureus strain FDA 209 (ATCC 6538). This whole-genome sequenced product can be used in PCR and other molecular biology applications.
Product category
Bacteria
Product type
Nucleic acid
Derived from
Staphylococcus aureus subsp. aureus FDA 209 (ATCC 6538)
Organism
Staphylococcus aureus
Type strain
No
Applications
Bioinformatics
Drug development
Food testing
Media testing
Next-generation sequencing
Quality control
Mass
5 μg
Product format
Dried
Shipping information
Stored in 1X TE buffer
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
Filtration Efficiency Testing
Detection of
Efficacy testing
Inhibition testing
Media testing
Preparatory test control
Quality control strain
Testing
Testing antimicrobial agents
Testing antimicrobial handwashing formulations
Testing disinfectants
Drug discovery
Food testing
Testing sanitizers
Testing bactericides
Quality control strain for Biosynth products

Characteristics

Comments

This preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction (PCR) process and other molecular biology applications.

Handling information

Handling procedure
Centrifuge tube prior to opening to prevent loss of pelleted material
  1. Rehydrate contents of vial with molecular grade H2O.
  2. Place vial at 37°C for 1 hour or at 2°C to 8°C overnight.
  3. For more complete rehydration and to fully recover DNA, incubate the sample overnight at 4°C while rocking; then incubate for 1 hour at 65°C.  Resuspending the dried DNA in ≥250 µL may give better results.
Handling notes

Genomic DNA is appropriate for PCR and other molecular biology applications.

Quality control specifications

Total amount
Total DNA by PicoGreen® measurement was found to be approximately 5 µg.
Integrity
Integrity of DNA was determined by electrophoresis on a 1% agarose gel stained with SYBR Safe™, and was found to be of high molecular weight.
Functional tests
Functional activity was confirmed by PCR amplification of the 16S ribosomal RNA gene.
Identity
Identity confirmed by sequencing of 16S ribosomal RNA gene (first ~500 base pairs).
Verification method
Whole-genome Sequencing

History

Deposited as
Staphylococcus aureus subsp. aureus Rosenbach
Depositors
ATCC
Chain of custody
ATCC <-- FDA <-- AMC
Cross references
GenBank DQ212950 Staphylococcus aureus strain ATCC 6538 16S ribosomal RNA gene, partial sequence.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Connolly P, et al. The use of impedance for preservative efficacy testing of pharmaceuticals and cosmetic products. J. Appl. Bacteriol. 76: 68-74, 1994. PubMed: 8144407

ASTM International StandardTest Method for Efficacy of Sanitizers Recommended for Inanimate Non-Food Contact Surfaces. West Conshohocken, PA

ASTM International Standard Test Method for Preservatives in Water-Containing Cosmetics. West Conshohocken, PA

AOAC International Germicidal and detergent sanitizing action of disinfectants. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 960.09.

AOAC International Bacteriostatic activity of laundry additive disinfectants. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 972.04.

View All Curated Citations for this Product

Frequently Asked Questions

Need assistance with this product? Contact our Technical Support team.

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