Brevibacillus laterosporus (Laubach) Shida et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate all tubes and plate at 30°C for 24 hours.
Vegetative cells are small to medium sized bacilli, occur individually and in pairs, and have a spinning-wheel motility.
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Smith NR, et al. Aerobic spore forming bacteria. US Dep Agric Monogr 16: 1-148, 1952.