Sporosarcina pasteurii (Miquel) Yoon et al.
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2. Using a single tube of #1376 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate. The cryoprotectant used in the freeze-drying procedure may inhibit growth in the primary tube, hence the necessity for immediate transfer.
5. Incubate all tubes and plate at 30oC for 48 to 72 hours. If growth is not heavy on agar medium, make additional transfers from the incubated broth at this time.
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Gibson T. An investigation of the Bacillus pasteuri group. III. Systematic relationships of the group. J. Bacteriol. 29: 491-502, 1935.
Smith NR, et al. Aerobic spore forming bacteria. US Dep Agric Monogr 16: 1-148, 1952.
Smith NR, et al. Aerobic mesophilic spore-forming bacteria. U.S. Dep. Agric. Misc. Publ. 559: 41, 1946.