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Fgf8 cDNA1 [mFgf8]

63351

Organism
Mus musculus, mouse
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Mission Collection Item
This is a Mission Collection Item.

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
To prepare an antisense probe, cut the plasmid with PstI and use T7 RNA polymerase.

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): SmaI/XhoI--3.0, 0.8; SmaI--3.8; XhoI--3.8; NcoI--3.65, 0.15; PstI--3.8.
The insert contains the following restriction sites (approximate kb from the 5' end): BalI--0.14; NcoI--0.16; StyI--0.16.
This insert is an alternate splice variant of Fgf8 that corresponds to bp 1-35 and 906-1038 of Z46883.
To prepare an antisense probe, cut the plasmid with PstI and use T7 RNA polymerase.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
3.799999952316284

Insert information

Insert size (kb)
0.80000000000000004
Type of DNA
cDNA
Gene product
fibroblast growth factor-8(androgen-induced growth factor) [AIGF]
Alleles
b, b, s, s

Handling information

History

Depositors
C Murphy, GR Martin
Cross references
GenBank Z46883

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

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Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Crossley PH, Martin GR. The mouse Fgf8 gene encodes a family of polypeptides and is expressed in regions that direct outgrowth and patterning in the developing embryo. Development 121: 439-451, 1995. PubMed: 7768185

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