ATCC 100 Years Logo Anniversary ATCC 100 Years Logo Anniversary Cart 0
  • Quick Order
  • Careers
  • Support

mMT-1

63146

Organism
Mus musculus, mouse
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Buy Now
Price: $639.00 EA
Discounts may be available for our fellow nonprofit organizations. Login to see your price.

Generally ships within 1-3 business days

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.3; BglI/BglII--2.2, 2.0, 1.4, 0.3 (doublet); EcoRI/HindIII--4.4, 1.9; KpnI--6.4; PstI--4.4, 1.9.
The insert contains the following restriction sites (approximate kb from the 5' end): KpnI--0.1; BstEII--0.4; BglI--0.57; BglII--0.82; PstI--1.1.
Please note that the promoter is the subject of issued U.S. Patent No. 4,579,821.
Corresponds to sequences from -750 to +1100 where +1 is the transcription start point.
The region between the BglI site (-185 or nt 116 of the published sequence) and the BglII site (+65 or nt 369 of the published sequence) contains the entire metal-responsive promoter.
The vector is a high copy number derivative of pBR322 with a XhoI linker at about 3000 on the standard map).
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
6.300000190734863

Insert information

Insert size (kb)
1.8500000000000001
Type of DNA
genomic
Insert source
embryo
Insert tissue
embryo
Gene product
metallothionein 1A (functional)(metallothionein 1, metallothionein 1A) [Mt1]
Alleles
b, a

Handling information

History

Depositors
RD Palmiter
Cross references
GenBank V00835

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Durnam DM, et al. Isolation and characterization of the mouse metallothionein-I gene. Proc. Natl. Acad. Sci. USA 77: 6511-6515, 1980. PubMed: 6935664

Glanville N, et al. Structure of mouse metallothionein-I gene and its mRNA. Nature 292: 267-269, 1981. PubMed: 7254320

Palmiter RD, Brinster RL. Control of DNA sequence transcription. US Patent 4,579,821 dated Apr 1 1986

Scherer G, et al. Gene mapping on mouse chromosome 8 by interspecific crosses: new data on a linkage group conserved on human chromosome 16q. Genomics 5: 275-282, 1989. PubMed: 2571576

Fletcher C, et al. A multilocus linkage map of mouse chromosome 8. Genomics 9: 737-741, 1991. PubMed: 2037298

View All Curated Citations for this Product

For product-related inquiries and issues, contact Product Experience:

Message Us

Hours of Operation

Monday - Friday
9:00am - 5:00pm
US Eastern Time