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pGEM4Z/SEAP

61497

Organism
Homo sapiens
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
Rehydrate with TE
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
If the plasmid is linearized with HindIII, and transcribed from the SP6 promoter in the presence of m7-GpppG, mRNA for the secreted form of placental alkaline phosphatase is produced.
mRNA prepared from this construct can be used as an internal standard for expression of mRNA injected into Xenopus oocytes.

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; HindIII--5.2; BamHI--3.2, 1.8; PstI--3.2, 1.8; SalI--5.2; EcoRI/KpnI--2.9, 2.1.
If the plasmid is linearized with HindIII, and transcribed from the SP6 promoter in the presence of m7-GpppG, mRNA for the secreted form of placental alkaline phosphatase is produced.
mRNA prepared from this construct can be used as an internal standard for expression of mRNA injected into Xenopus oocytes.
Linkage has been established between Waardenburg syndrome type 1 and ALPP in five families (LOD score 4.76 at a recombination fraction of 0.023.
Waldenburg syndrome shows close linkage to FN1 and ALPP in one multigenerational family with 11 affected individuals.
The described RsaI polymorphism was established using a 1.1 kb EcoRI/ApaI fragment of the ALPP cDNA (ALP-1).
Constructed from pBC12/PLAP containing the complete cDNA for the membrane-anchored form by inserting a termination codon after the codon for amino acid 489. The EcoRI/KpnI fragment was then cloned into the vector.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
5.0

Insert information

Insert size (kb)
2
Type of DNA
cDNA
Gene product
alkaline phosphatase, placental (Regan isozyme) [ALPP]
Alleles
A1, A2, B1, B2

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Berger J, et al. Secreted placental alkaline phosphatase: a powerful new quantitative indicator of gene epxression in eukaryotic cells. Gene 66: 1-10, 1988. PubMed: 3417148

Tate SS, et al. Secreted alkaline phosphatase: an internal standard for expression of injected mRNAs in the Xenopus oocyte. FASEB J. 4: 227-231, 1990. PubMed: 2298343

Penhallow RC, Sussman HH. Further characterization of SacI RFLPs at the acid alpha glucosidase (GAA) locus. Nucleic Acids Res. 18: 1930, 1990. PubMed: 1970872

Foy C, et al. Assignment of the locus for Waardenburg syndrome type 1 to human chromosome 2q37 and possible homology to the splotch mouse. Am. J. Hum. Genet. 46: 1017-1023, 1990. PubMed: 2339698

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