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pA10RP8 [pA10RP8]

61396

Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
Escherichia coli containing the plasmid
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): SacI--3.0, 1.35; SacI/PstI--3.0, 0.9, 0.4; PstI--3.8, 0.6; EcoRI--3.36, 0.38 (doublet), 0.3; HindIII--4.3.
There is tight linkage between multiple endocrine neoplasia type 2A (MEN2A) and D10Z1 (theta = 0, LOD score = 12.02).
Data for haplotypes formed by using the A and F morphs improved the LOD score for linkage between multiple endocrine neoplasia type 2A (MEN2A) and D10Z1 (theta = 0, LOD score = 14.06).
There is tight linkage between medullary thyroid carcinoma (MTC) with parathyroid tumors (PTs) and no pheochromocytomas (PHEOs) and D10Z1 (theta = 0, LOD score = 2.86).
The insert contains 4 EcoRI repeat units of 340 bp each. A SacI/PstI double digest should give fragments of 1.0 kb and 0.36 kb from the insert.
High stringency hybridization and washing conditions are necessary due to the repetitive nature of the probe.
The HinfI polymorphism detects the haplotypes at the following frequencies: F+G+ (0.5), F+G- (not seen), F-G+ (0.02), and F-G- (0.48).
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
4.300

Insert information

Insert size (kb)
1.3500000000000001
Gene product
DNA Segment, repetitive [D10Z1]
Alleles
A2, B2, F2, G2, A2, B2, F2, G2, A1, B1, F1, G1, A1, B1, F1, G1

Handling information

Host
Escherichia coli HB101 (ATCC 33694)
Medium
Temperature
37°C

History

Depositors
P Devilee
Cross references
GenBank 175196

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Devilee P, et al. Chromosome-specific alpha satellite DNA: isolation and mapping of a polymorphic alphoid repeat from human chromosome 10. Genomics 3: 1-7, 1988. PubMed: 3220475

Wu JS, et al. The genetic defect in multiple endocrine neoplasia type 2A maps next to the centromere of chromosome 10. Am. J. Hum. Genet. 46: 624-630, 1990. PubMed: 1968709

Carson NL, Simpson NE. Two HinfI RFLPs detected by palphaRP8 at D10Z1. Nucleic Acids Res. 18: 1932, 1990. PubMed: 1970876

Carson NL, et al. The mutation for medullary thyroid carcinoma with parathyroid tumors (MTC with PTs) is closely linked to the centromeric region of chromosome 10. Am. J. Hum. Genet. 47: 946-951, 1990. PubMed: 1978560

Peter Devilee, personal communication

Frequently Asked Questions

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