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phgJE3-3

61366

Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
The insert contains the following sites (with nucleotide positions according to the published sequence): NaeI at -426; HindIII at -420; SacI at -408 and PstI at -307.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.8; HindIII--3.65, 0.15; BamHI--3.8; PstI--3.8; XbaI/SacI--2.9, 0.7, 0.2.
The 5' clone phgJE3-3 (ATCC 61366/61367) and the 3' clone phgJE2-1 (ATCC 61368/61369) together generate a complete gene.
This insert was subcloned from lambdahJE-7 and extends from -531 relative to the mRNA cap site into the first intron which begins as +228. It is oriented in this clone with transcription from the SP6 promoter giving the + strand.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
3.799999952316284

Insert information

Insert size (kb)
0.80000000000000004
Type of DNA
genomic
Insert source
embryonic lung fibroblast WI-38 (serum-induced)
Insert tissue
embryonic lung fibroblast WI-38 (serum-induced)
Gene product
cytokine A2, small inducible( monocyte chemotactic protein, monocyte chemotactic protein 1, homologous to mouse Sig-je, small inducible cytokine A2, small inducible cytokine subfamily A (Cys-Cys), member 2, small inducible gene JE) [JE*]

Handling information

History

Depositors
BJ Rollins
Cross references
GenBank M26035
GenBank 173478

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Rollins BJ, et al. The human homolog of the JE gene encodes a monocyte secretory protein. Mol. Cell. Biol. 9: 4687-4695, 1989. PubMed: 2513477

Barrett J Rollins, personal communication

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