ATCC ATCC Logo 0

HD2G1

61355

Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
Rehydrate with TE

Documentation

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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
Codominant inheritance was observed in 3 informative families (39 children).

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 9.0, 5.0, 4.3, 1.8, 1.7, 1.7, 0.7; BglII--25.0, 7.0, 3.2, 3.0, 2.4, 1.5, 0.68; PvuI--20.0, 12.0, 8.0; HindIII--21.0, 18.0, 4.3, 0.8; SmaI--20.0, 19.0, 5.6.
The insert contains the following sites separated by (kb)(approx): SalI, MboII- 1.5- BamHI- 1.7- BamHI- 1.7- BamHI- 4.3- BamHI- 1.8- BamHI- 0.65- BamHI- 5.0- SmaI- 0.6- BamHI- 0.7- MboII, SalI.
Codominant inheritance was observed in 3 informative families (39 children).
The 1.6 kb BamHI fragment contains exon 7 and 3' sequences. Exon 7, which has been sequenced, encodes the last 64 amino acids and includes 1.2 kb of 3' non-coding sequence.
The BamHI 1.6 doublet detects the TaqI RFLP associated with DRD2.
Use standard hybridization conditions with the addition of human placental DNA to block repeat sequences. Washing conditions are standard with a final wash at 0.1 x SSC, 0.1% SDS, 65C.
Enzyme(s) not detecting polymorphism: 31 enzymes.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
44.0

Insert information

Insert size (kb)
16
Type of DNA
genomic
Insert source
male
Insert tissue
male
Gene product
dopamine receptor D2 [DRD2]
Alleles
A1, B1, B2, A2

History

Cross references
GenBank M29066

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
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Disclaimers

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While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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References

Curated Citations

Grandy DK, et al. The human dopamine D2 receptor gene is located on chromosome 11 at q22-q23 and identifies a TaqI RFLP. Am. J. Hum. Genet. 45: 778-785, 1989. PubMed: 2573278

Grandy DK, et al. Cloning of the cDNA and gene for a human D2 dopamine receptor. Proc. Natl. Acad. Sci. USA 86: 9762-9766, 1989. PubMed: 2532362

Olivier Civelli, personal communication

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