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puc19TM15 [lambda HTm15]

61349

Organism
Homo sapiens
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
Rehydrate with TE
Mission Collection Item
This is a Mission Collection Item

Documentation

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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
The insert contains the following sites separated by (bp)(approx): EcoRI- 270- SmaI- 160- PstI- 240- PstI- 210- PstI- 100- PstI- 530- KpnI- 310- PstI- 1080- HindIII- 110- BamHI- 205- PstI- 475- EcoRI.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 2.7; SacI--6.5; AvaI--3.4, 2.0, 0.2; XbaI--5.9, 0.54, 0.2, 0.1.
The insert includes 146 nt of 5'-noncoding sequence, an open reading frame of 1725 nt and 1779 nt of 3'-noncoding sequence including 40 nt of poly(A) tail.
There is 64% homology between this probe and bovine thrombomodulin.
A single band of 3.7 kb is detected in human placenta and endothelial cell poly(A)+ RNA.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
6.400000095367432

Insert information

Insert size (kb)
3.7000000000000002
Type of DNA
cDNA
Insert source
umbilical vein endothelial cells
Insert tissue
umbilical vein endothelial cells
Gene product
thrombomodulin [THBD]

History

Cross references
GenBank M16552

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Wen DZ, et al. Human thrombomodulin: complete cDNA sequence and chromosome localization of the gene. Biochemistry 26: 4350-4357, 1987. PubMed: 2822087

Espinosa R, et al. Regional localization of the human thrombomodulin gene to 20p12-cen. Genomics 5: 649-650, 1989. PubMed: 2559025

Scarpati EM, Sadler JE. Regulation of endothelial cell coagulant properties. J. Biol. Chem. 264: 20705-20713, 1989. PubMed: 2555368

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