ATCC 2023 Holiday Shipping Schedule: We will be closed from December 22nd to January 1st. To ensure timely delivery, please submit orders in accordance with the following: Domestic orders no later than December 15th for shipment by December 20th 2023; International orders no later than December 12th for shipment by the first week of January 2024. We will resume our normal shipping schedule on January 2nd, 2024. Thank you for your cooperation. 

  • Quick Order
  • Careers
  • Support

pE25.a [pBS300]


The human repetitive DNA segment (D1Z7) from the hamster/human XJM12.1.3 hybrid cell line. The first half of the dimer is 85% identical to the sequence of the 1’ monomer: the second half is 80% identicial to the 2’ monomer (1).  The insert is a dimer of the 171 bp consensus sequence of the alphoid DNA from the subfamily of an alpha-satellite DNA made up of EcoR I dimers or multiples thereof. The genomic DNA was cloned as an EcoR I fragment into pBS vector, transformed into Escherichia coli HB101 (ATCC 33694) and provided as a freeze-dried culture.
Clone type
Molecular biology
Product format
Shipping information
Escherichia coli containing the plasmid
Storage conditions
2°C to 8°C
Mission Collection Item
This is a Mission Collection Item.


ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information


The insert contains the following sites (in bp from an end): Sau3A (20), HaeIII(52), PstI (85), HinfI (181), HaeIII (227).
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 0.35; BamHI--3.6; HindIII--3.6; KpnI--3.6; HincII--3.6.
The first half of the dimer is 85% identical to the sequence of the 1' monomer; the second half is 80% identical to the 2' monomer.
The insert is a dimer of the 171 bp consensus sequence of alphoid DNA from the subfamily of an alpha-satellite DNA made up of EcoRI dimers or multiples thereof.
Mycoplasma contamination
Not detected

Vector information

Intact vector size
Vector name
Cloning sites
EcoRI; SacI; KpnI; SmaI; BamHI; XbaI; SalI; PstI; SphI; HindIII
Lac, T3, T7
pUC (pMB1)

Insert information

Insert size (kb)
Insert source
DNA from hamster/human XJM12.1.3 hybrid cell line
Insert tissue
DNA from hamster/human XJM12.1.3 hybrid cell line
Gene product
DNA Segment, repetitive [D1Z7]

Handling information

Escherichia coli HB101 (ATCC 33694)
Handling procedure
Aseptically add to the freeze-dried material 0.3 to 0.4 mL (no more) of liquid medium and mix well. Transfer 100 µl to a test tube containing 5 mL LB-ampicillin.  A loopful of the culture can be streaked on an LB-ampicillin agar plate.  Incubate cultures at 37°C.   Isolate DNA using standard plasmid preparation procedures (2).   Digestion of the plasmid with EcoR I results in bands at 3.2 kb and 0.35 kb.  Digestion of the plasmid with either Kpn I, BamH I, Hind III, or Hinc II results in a band at 3.6 kb.  The insert contains the following sites (in bp from an end):  Sau 3A (20), Hae III (52), Pst I (85), Hinf I (181) and Hae III (227).


IE Scheffler
Cross references
GenBank 173483

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Carine K, et al. Molecular characterization of human minichromosomes with centromere from chromosome 1 in human-hamster hybrid cells. Somatic Cell Mol. Genet. 15: 445-460, 1989. PubMed: 2781415

Immo E Scheffler, personal communication

Need assistance with this product? Contact our Technical Support team.


US and Puerto Rico

Outside the US

Hours of Operation

9:00am - 5:00pm
US Eastern Time