pE25.a [pBS300]

61304 ^™

The human repetitive DNA segment (D1Z7) from the hamster/human XJM12.1.3 hybrid cell line. The first half of the dimer is 85% identical to the sequence of the 1’ monomer: the second half is 80% identicial to the 2’ monomer (1). The insert is a dimer of the 171 bp consensus sequence of the alphoid DNA from the subfamily of an alpha-satellite DNA made up of EcoR I dimers or multiples thereof. The genomic DNA was cloned as an EcoR I fragment into pBS vector, transformed into Escherichia coli HB101 (ATCC 33694) and provided as a freeze-dried culture.

Clone type: Clone
Applications: Molecular biology
Product format: Freeze-dried
Shipping information: Escherichia coli containing the plasmid
Storage conditions: 2°C to 8°C

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Documentation

Product sheet

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Characteristics

Comments: The insert contains the following sites (in bp from an end): Sau3A (20), HaeIII(52), PstI (85), HinfI (181), HaeIII (227).
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 0.35; BamHI--3.6; HindIII--3.6; KpnI--3.6; HincII--3.6.
The first half of the dimer is 85% identical to the sequence of the 1' monomer; the second half is 80% identical to the 2' monomer.
The insert is a dimer of the 171 bp consensus sequence of alphoid DNA from the subfamily of an alpha-satellite DNA made up of EcoRI dimers or multiples thereof.
Mycoplasma contamination: Not detected

Vector information

Intact vector size: 3.2
Vector name: pE25.a
Cloning sites: EcoRI; SacI; KpnI; SmaI; BamHI; XbaI; SalI; PstI; SphI; HindIII
Markers: ampR
Promoters: Lac, T3, T7
Replicon: pUC (pMB1)

Insert information

Insert size (kb): 0.34899999999999998
Insert source: DNA from hamster/human XJM12.1.3 hybrid cell line
Insert tissue: DNA from hamster/human XJM12.1.3 hybrid cell line
Gene product: DNA Segment, repetitive [D1Z7]

Handling information

Host: Escherichia coli HB101 (ATCC 33694)
Medium: ATCC Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Temperature: 37°C
Handling procedure: Aseptically add to the freeze-dried material 0.3 to 0.4 mL (no more) of liquid medium and mix well. Transfer 100 µl to a test tube containing 5 mL LB-ampicillin. A loopful of the culture can be streaked on an LB-ampicillin agar plate. Incubate cultures at 37°C. Isolate DNA using standard plasmid preparation procedures (2). Digestion of the plasmid with EcoR I results in bands at 3.2 kb and 0.35 kb. Digestion of the plasmid with either Kpn I, BamH I, Hind III, or Hinc II results in a band at 3.6 kb. The insert contains the following sites (in bp from an end): Sau 3A (20), Hae III (52), Pst I (85), Hinf I (181) and Hae III (227).

History

Depositors: IE Scheffler
Cross references: GenBank 173483

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

References

Curated Citations

Carine K, et al. Molecular characterization of human minichromosomes with centromere from chromosome 1 in human-hamster hybrid cells. Somatic Cell Mol. Genet. 15: 445-460, 1989. PubMed: 2781415

Immo E Scheffler, personal communication

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