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pUC-Cathepsin G [pUC-Cathepsin]

61245

Organism
Homo sapiens
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
Rehydrate with TE
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
This insert includes an internal HindIII site which maps to exon 5.
Restriction digests of the clone give the following sizes (kb): BamHI--5.7; HindIII--3.0, 2.7; EcoRI--3.0, 2.7; HincII--5.7.
A unique probe for cathepsin G can be made by purifying the 0.35 kb HindIII/EcoRI fragment derived from exon 5 and the 3' flanking region of this gene.
The insert is an EcoRI fragment that begins at position -76 with respect to the transcription initiation site and ends about 300 bp downstream of the poly(A) site.
The cathepsin G gene is divided into 5 exons and 4 introns, similar to several other serine proteases, including human neutrophilelastase, rat mast cell protease II, murine adipsin and murine and human cytotoxic T-cell serine proteases.
At high stringency, this probe hybridizes to a genomic BamHI fragment of 6.0 kb, a genomic BglII fragment of 8.5 kb, and a genomic EcoRI fragment of about 3.0 kb. There is also a polymorphic 4.3 kb BglII fragment.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
5.699999809265137

Insert information

Insert size (kb)
3
Type of DNA
genomic
Insert source
lung fibroblast
Insert tissue
lung fibroblast
Gene product
cathepsin G [CTSG]

History

Cross references
GenBank J04990

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Hohn PA, et al. Genomic organization and chromosomal localization of the human cathepsin G gene. J. Biol. Chem. 264: 13412-13419, 1989. PubMed: 2569462

Hanson RD, et al. Developmental regulation of the human cathepsin G gene in myelomonocytic cells. J. Biol. Chem. 265: 1524-1530, 1990. PubMed: 2295643

Timothy J Ley, personal communication

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