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Y-190 [Y190]

61166

Organism
Homo sapiens
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
This probe is useful for detection of Y-derived marker chromosomes and mosaicism.

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 3.5; EcoRI--6.1, 1.8; AvaI--4.7, 3.2; BamHI--8.0; PvuII--8.0.
The sequences detected by Y-190 are present in numerous XX males.
This probe hybridizes to Y-specific HindIII fragments of about 4 and 5 kb (strong) and 9 kb (weak) in the orangutan and 4 kb and 9 kb (weak) in the rhesus monkey.
This probe detects 2 blocks of repeats on Yp. The hypervariable major block is composed of tandem 20.3 kb repeats with alleles ranging from about 540 to 800 kb (XbaI). The minor block is not variable and is about 60 kb.
Detects a Y-specific 3.5 kb HindIII fragment that has a copy number of about 100 in normal human males. Also detects less repeated HindIII fragments ranging from 1.5 to 5.7 kb.
This probe is useful for detection of Y-derived marker chromosomes and mosaicism.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
7.90
Intact vector size
4.363
Vector name
pBR322
Type of vector
plasmid
Construction
pBR313
Vector end
HindIII
Vector information
Cross references: DNA Seq. Acc.: J01749
Cloning sites
EcoRI; ClaI; HindIII; EcoRV; BamHI; SphI; SalI; XmaIII; NruI; BspMI; BsmI; StyI; AvaI; BalI; BspMII; PvuII; Tth111I; NdeI; AflIII; PpaI; PstI; PvuI; ScaI; SspI; AatII
Markers
ampR; tetR
Replicon
pMB1

Insert information

Insert size (kb)
3.5
Type of DNA
genomic
Insert source
49,XXXXY cell line
Insert tissue
49,XXXXY cell line
Insert information
Genomic copy number: repetitive
Type of nucleic acid: genomic
Restriction enzyme: XbaI
Size range (kb): 550-700
Type of polymorphism: VNTR
Genome
Homo sapiens
Chromosome
Y
Y p
Gene name
DNA Segment, repetitive
Gene symbol
DYZ5
Contains complete coding sequence
Unknown
Insert end
HindIII

Handling information

Host
Escherichia coli HB101 (ATCC 33694)
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 3.5; EcoRI--6.1, 1.8; AvaI--4.7, 3.2; BamHI--8.0; PvuII--8.0.
- ATCC staff

The sequences detected by Y-190 are present in numerous XX males. This probe detects 2 blocks of repeats on Yp. The hypervariable major block is composed of tandem 20.3 kb repeats with alleles ranging from about 540 to 800 kb (XbaI). The minor block is not variable and is about 60 kb.
- GDB

This probe is useful for detection of Y-derived marker chromosomes and mosaicism.
- Hum. Genet. 75: 109-113, 1987

This probe hybridizes to Y-specific HindIII fragments of about 4 and 5 kb (strong) and 9 kb (weak) in the orangutan and 4 kb and 9 kb (weak) in the rhesus monkey.
- Development 101: 51-58, 1987 (supplement)

Detects a Y-specific 3.5 kb HindIII fragment that has a copy number of about 100 in normal human males. Also detects less repeated HindIII fragments ranging from 1.5 to 5.7 kb.
- Nucleic Acids Res. 14: 6489-6505, 1986

History

Depositors
U Muller, Institute of Human Genetics
Cross references
GenBank 165712

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Muller U, et al. Deletion mapping of the testis determining locus with DNA probes in 46,XX males and in 46,XY and 46,X,dic(Y) females. Nucleic Acids Res. 14: 6489-6505, 1986. PubMed: 3748818

Gaunt SJ. Mapping of testis-determining locus on Yp by the molecular genetic analysis of XX males and XY females. Development 101: 51-58, 1987. PubMed: 88211484

Muller U, et al. Y-190, a DNA probe for the sensitive detection of Y-derived marker chromosomes and mosaicism. Hum. Genet. 75: 109-113, 1987. PubMed: 3469135

Tyler-Smith C, et al. Structure of a hypervariable tandemly repeated DNA sequence on the short arm of the human Y chromosome. J. Mol. Biol. 203: 837-848, 1988. PubMed: 3210241

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