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vW-8 [pvW]

59127

Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Frozen
Shipping information
Rehydrate with TE
Mission Collection Item
This is a Mission Collection Item.

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
There seems to be a partial pseudogene for VWF detected by sequences from about 2.5-4.5 kb of the cDNA. For unique sequence probes from the 5' end, EcoRI/HindIII or EcoRI/BglII or EcoRI/XmaIII fragments are useful.
For unique sequence probes from the 3' end, the fragment from NarI or XmnI to SalI (in the polylinker) is useful.

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--9.4, 2.9; BamHI--5.8, 5.8; HindIII--6.6, 5.3; PstI--3.4, 1.8, 1.1, 0.8, + others;
purified DNA: HindIII--6.64, 5.7; SacI--6.6, 3.8, 2.0; BamHI--6.2 doublet; EcoRI--14; EcoRI/SacI--3.8, 3.4, 3.0. 1.0.
The insert contains the following singly cutting sites: BamHI, EcoRV, HgaI, HindIII, MspI, NaeI, PvuI, RshI, Tth111II, XbaI, XmaIII, XmnI, XorII.
The following enzymes do not cut vW-8: AhaIII, AsuII, BclI, BssHII, HpaI, MluI, NdeI, NotI, NruI, SalI, SfiI, SnaBI.
This is essentially a full-length cDNA clone except for about 150 bp 5' untranslated and 100 bp 3' untranslated sequence.
There seems to be a partial pseudogene for VWF detected by sequences from about 2.5-4.5 kb of the cDNA. For unique sequence probes from the 5' end, EcoRI/HindIII or EcoRI/BglII or EcoRI/XmaIII fragments are useful.
For unique sequence probes from the 3' end, the fragment from NarI or XmnI to SalI (in the polylinker) is useful. Sequences at the 3' end show homology to vole, rat, hamster, monkey, and mouse but not quail.
Codominant segregation for the TaqI polymorphism has been demonstrated in 4 families.
The BamHI polymorphism is detected with a 1.9 kb SacI fragment of the cDNA.
The EcoRI polymorphism is detected by a 3.6 kb XmnI/SalI fragment of this clone.
Enzyme(s) not detecting polymorphism (for the 1.9 kb SacI fragment probe): AvaI, BglII, BstEII, DraI, EcoRI, HindIII, KpnI, PstI (tested in 13-15 unrelated Caucasians).
Enzyme(s) not detecting polymorphism: BglII, EcoRI, SacI, TaqI.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
12.0

Insert information

Insert size (kb)
8.8000000000000007
Type of DNA
cDNA
Insert source
endothelium
Insert tissue
endothelium
Gene product
von Willebrand factor pseudogene [VWF]
Alleles
K2, M1, A4, C1, C2, D1, D2, G1, G2, K1, K2, M1, M1, M2, A1, A2, A3, A5, A6, A7, A4, C1, C2, D1, D2, G1, G2, K1, M1, M2, A1, A2, A3, A5, A6, A7, M2, M2, , , , A1, A2, A1, A1, A2, A2

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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Frequently Asked Questions

References

Curated Citations

Marchetti G, et al. Two additional TaqI RFLPs in von Willebrand factor gene (VWF) and pseudogene. Nucleic Acids Res. 17: 3329, 1989. PubMed: 2566970

Ewerhardt B, et al. An EcoRI polymorphism in the human von Willebrand factor (vWF) gene. Nucleic Acids Res. 17: 5416, 1989. PubMed: 2569713

Driscoll CM, et al. A KpnI DNA polymorphism in the human von Willebrand factor (VWF) gene. Nucleic Acids Res. 18: 4968, 1990. PubMed: 1975687

Bernardi F, et al. Two TaqI RFLPs in the human von Willebrand factor gene. Nucleic Acids Res. 15: 1347, 1987. PubMed: 2881274

Nishino K, Lynch DC. A polymorphism of the human von Willebrand factor (vWF) gene with BamHI. Nucleic Acids Res. 14: 4697, 1986. PubMed: 3012477

View All Curated Citations for this Product

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