vW-8 [pvW]

59127 ^™

90/100

Bioz Stars

1 Product Citation

Organism: Homo sapiens, human
Clone type: Clone
Applications: Molecular biology
Product format: Frozen
Shipping information: Rehydrate with TE

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Documentation

Product sheet

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

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General

Specific applications: There seems to be a partial pseudogene for VWF detected by sequences from about 2.5-4.5 kb of the cDNA. For unique sequence probes from the 5' end, EcoRI/HindIII or EcoRI/BglII or EcoRI/XmaIII fragments are useful.
For unique sequence probes from the 3' end, the fragment from NarI or XmnI to SalI (in the polylinker) is useful.

Characteristics

Comments: Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--9.4, 2.9; BamHI--5.8, 5.8; HindIII--6.6, 5.3; PstI--3.4, 1.8, 1.1, 0.8, + others;
purified DNA: HindIII--6.64, 5.7; SacI--6.6, 3.8, 2.0; BamHI--6.2 doublet; EcoRI--14; EcoRI/SacI--3.8, 3.4, 3.0. 1.0.
The insert contains the following singly cutting sites: BamHI, EcoRV, HgaI, HindIII, MspI, NaeI, PvuI, RshI, Tth111II, XbaI, XmaIII, XmnI, XorII.
The following enzymes do not cut vW-8: AhaIII, AsuII, BclI, BssHII, HpaI, MluI, NdeI, NotI, NruI, SalI, SfiI, SnaBI.
This is essentially a full-length cDNA clone except for about 150 bp 5' untranslated and 100 bp 3' untranslated sequence.
There seems to be a partial pseudogene for VWF detected by sequences from about 2.5-4.5 kb of the cDNA. For unique sequence probes from the 5' end, EcoRI/HindIII or EcoRI/BglII or EcoRI/XmaIII fragments are useful.
For unique sequence probes from the 3' end, the fragment from NarI or XmnI to SalI (in the polylinker) is useful. Sequences at the 3' end show homology to vole, rat, hamster, monkey, and mouse but not quail.
Codominant segregation for the TaqI polymorphism has been demonstrated in 4 families.
The BamHI polymorphism is detected with a 1.9 kb SacI fragment of the cDNA.
The EcoRI polymorphism is detected by a 3.6 kb XmnI/SalI fragment of this clone.
Enzyme(s) not detecting polymorphism (for the 1.9 kb SacI fragment probe): AvaI, BglII, BstEII, DraI, EcoRI, HindIII, KpnI, PstI (tested in 13-15 unrelated Caucasians).
Enzyme(s) not detecting polymorphism: BglII, EcoRI, SacI, TaqI.
Mycoplasma contamination: Not detected

Vector information

Construct size (kb): 12.0

Insert information

Insert size (kb): 8.8000000000000007
Type of DNA: cDNA
Insert source: endothelium
Insert tissue: endothelium
Gene product: von Willebrand factor pseudogene [VWF]
Alleles: K2, M1, A4, C1, C2, D1, D2, G1, G2, K1, K2, M1, M1, M2, A1, A2, A3, A5, A6, A7, A4, C1, C2, D1, D2, G1, G2, K1, M1, M2, A1, A2, A3, A5, A6, A7, M2, M2, , , , A1, A2, A1, A1, A2, A2

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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