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pHU10 plasmid in E. coli

57002

This clone contains a 2.8 kb human genomic DNA fragment cloned into the HindIII site of pBR322. The insert fragment maps to chromosome 13q12.3; D13S6. An unpublished RFLP is detected by this probe when genomic DNA is digested with EcoRI.  The published RFLP is revealed with XmnI. A multiple site polymorphism is detected with XmnI and EcoRI, with a common band of 6.0 and alleles of 8.5 and 10 kb.
Organism
Homo sapiens, human
Clone type
Vector
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
Escherichia coli containing the plasmid
Storage conditions
2°C to 8°C
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 2.8; PstI--7.2.
A multiple site polymorphism also detected with XmnI and EcoRI, with a common band of 6.0 and alleles of 8.5 and 10 kb.
An unpublished RFLP is detected by this probe when genomic DNA is digested with EcoRI. The published RFLP is revealed with XmnI.
Mycoplasma contamination
Not detected

Vector information

Vector name
pBR322
Markers
cmlR; kanR; ampR; URA3

Insert information

Insert size (kb)
2.7999999999999998
Insert information
Gene: DNA segment, single copy
Insert end
HindIII

Handling information

Host
Escherichia coli K12
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone gave the following sizes (in kb): HindIII - 4.4, 2.8 ; PstI - 7.2.
-ATCC Staff

History

Depositors
TP Dryja
Cross references
GenBank 164538

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Dryja TP, et al. Chromosome 13 restriction fragment length polymorphisms. Hum. Genet. 65: 320-324, 1984. PubMed: 6319270

Dryja TP, Morton CC. Mapping of seven polymorphic loci on human chromosome 13 by in situ hybridization. Hum. Genet. 71: 192-195, 1985. PubMed: 4065891

Misra BC, Srivatsan ES. Localization of HeLa cell tumor-suppressor gene to the long arm of chromosome II. Am. J. Hum. Genet. 45: 565-577, 1989. PubMed: 2577469

Lundberg C, et al. Loss of heterozygosity in human ductal breast tumors indicates a recessive mutation on chromosome 13. Proc. Natl. Acad. Sci. USA 84: 2372-2376, 1987. PubMed: 3031679

Leppert M, et al. A primary genetic map of chromosome 13q. Am. J. Hum. Genet. 39: 425-437, 1986. PubMed: 3464200

View All Curated Citations for this Product

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