Kluyveromyces lactis (Dombrowski) van der Walt
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2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; overnight rehydration is recommended.
5. Mix the suspension well. Use several drops to inoculate recommended solid or liquid medium.
6. Incubate at recommended temperature.
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Shehata ET, et al. Yeasts isolated from Drosophila and their suspected feeding places in southern and central California. Mycologia 47: 799-811, 1955.
Falcone C, et al. Analysis of a 1.6-micron circular plasmid from the yeast Kluyveromyces drosophilarum: structure and molecular dimorphism. Plasmid 15: 248-252, 1986. PubMed: 3012617
Siderberg DG, Lachance MA. Speciation, species delineation, and electrophoretic isoenzyme patterns of the type strains of Kluyveromyces van der Walt emend. van der Walt. Int. J. Syst. Bacteriol. 33: 822-828, 1983.
Chen XJ, et al. A gene-cloning system for Kluyveromyces lactis and isolation of a chromosomal gene required for killer toxin production. J. Basic Microbiol. 28: 211-220, 1988. PubMed: 3193361
type strain of Saccharomyces drosophilarum