Sphingobacterium antarcticum Shivaji et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
5. Incubate all tubes and plate at 26°C for 48 hours.
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Rao NS, et al. Sphingobacterium antarcticus sp. nov., a psychrotrophic bacterium from the soils of Schirmacher Oasis, Antarctica. Int. J. Syst. Bacteriol. 42: 102-106, 1992.
Euzeby JP. Taxonomic note: necessary correction of specific and subspecific epithets according to Rules 12c and 13b of the International Code of Nomenclature of Bacteria (1990 Revision). Int. J. Syst. Bacteriol. 48: 1073-1074, 1998.