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Brachyspira alvinipulli Stanton et al.

51933

Type strain
Product category
Bacteria
Strain designation
91-1207/C1 [C1]
Type strain
Yes
Isolation source
Chicken cecum
Geographical isolation
United States; Ohio
Product format
Frozen
Storage conditions
-80°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Characterization
Preceptrol
No

Handling information

Medium
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
  1. Thaw vial at room temperature.  Under anaerobic conditions, aseptically withdraw entire contents of vial and transfer into a tube of #1827 broth.
  2. Additional tubes of #1827 broth or slants can be inoculated with 0.5 ml each of the suspension, making a biphasic culture in the slants.  Inoculate two sheep blood agar plates.
  3. Incubate the tubes under an anaerobic atmosphere at 37°C for 2-3 days.  One of the sheep blood agar plates is incubated under anaerobic conditions and the second plate is incubated aerobically to check for purity.
  4. Growth can be observed as light turbidity in the broth and as a thin film on the slants and #260 plate. Viable growth can also be observed by looking for actively motile helical cells when examining a wet mount of a drop from the broth portion of the biphasic slant under phase contrast.  The aerobic plate should show no signs of growth.

 

ANAEROBIC CONDITIONS:

Anaerobic conditions for transfer may be obtained by either of the following:

  • Use of an anaerobic gas chamber, or
  • Placement of test tubes under a gassing cannula system hooked to anaerobic gas.

 

Anaerobic conditions for incubation may be obtained by any of the following:

  • Loose screw caps on test tubes in anaerobic chamber,
  • Loose screw caps on test tubes in an activated anaerobic gas pack jar, or   
  • Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.  
Handling notes
The cells are motile helical rods in singles.
Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

Deposited as
Brachyspira alvinipulli Stanton et al.
Depositors
NS Jensen
Chain of custody
ATCC <-- NS Jensen <-- D.E. Swayne
Type of isolate
Animal

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Swayne DE, et al. Identification of a new intestinal spirochete with pathogenicity for chickens. Infect. Immun. 63: 430-436, 1995. PubMed: 7822007

Swayne DE, et al. Association of cecal spirochetes with pasty vents and dirty eggshells in layers. Avian Dis. 36: 776-781, 1992. PubMed: 1417614

Ochiai S, et al. Unification of the genera Serpulina and Brachyspira, and proposal of Brachyspira hyodysenteriae comb nov., Brachyspira innocens comb. nov. and Brachyspira pilosicoli comb. nov.. Microbiol. Immunol. 41: 445-452, 1997. PubMed: 9251055

Stanton TB, et al. Serpulina alvinipulli sp. nov., a new Serpulina species that is enteropathogenic for chickens. Int. J. Syst. Bacteriol. 48: 669-676, 1998. PubMed: 9734021

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