Hyphomicrobium denitrificans Urakami et al.
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2. From a single tube of #784 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well
4. Use several drops of this suspension to inoculate a second tube of broth, a slant and/or a plate.
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Harder W, et al. Methanol assimilation by Hyphomicrobium sp.. J. Gen. Microbiol. 78: 155-163, 1973.
Sasaki J, et al. Characterization and description of Hyphomicrobium denitrificans sp. nov.. Int. J. Syst. Bacteriol. 45: 528-532, 1995.