Tenacibaculum ovolyticum (Hansen et al.) Suzuki et al.
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2. Using a single tube of #2 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an agar slant and/or plate.
5. Incubate at 15oC for 48-72 hours.
Cells are Gram negative, slender, long rods. Culture will not grow above 20oC.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Hansen GH, et al. Flexibacter ovolyticus sp. nov., a pathogen of eggs and larvae of Atlantic halibut, Hippoglossus hippoglossus L. Int. J. Syst. Bacteriol. 42: 451-458, 1992. PubMed: 1503974