Rhodococcus opacus Klatte et al.
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2. Using a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #18 agar slant and/or plate.
5. Incubate the tubes and plate at 26°C for 7 days.
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Validation list no. 52. Int. J. Syst. Bacteriol. 45: 197-198, 1995.
Klatte S, et al. Rhodococcus opacus sp.nov., an unusual nutritionally versatile Rhodococcus-species. Syst. Appl. Microbiol. 17: 355-360, 1994.