Enterococcus faecium (Orla-Jensen) Schleifer and Kilpper-Balz
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2. From a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1 ml with a Pasteur or 1.0 ml pipette. Rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #44 agar slant and/or plate.
5. Incubate all tubes and plate at 37°C for 24 hours.
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Yohda M, et al. Molecular cloning and nucleotide sequencing of the aspartate racemase gene from lactic acid bacteria Streptococcus thermophilus. Biochim. Biophys. Acta 1089: 234-240, 1991. PubMed: 2054383